Chemical Biology Seminar Series, SciLifeLab Stockholm

Next Seminar: 2019-05-23, 15:30

''iPS cell models of human brain development, in health and disease''

Speaker: Anna Falk

Associate professor at the department of Neuroscience, Karolinska Institutet

''Role of RNA in DNA repair and chromatin structure''

Speaker: Marianne Farnebo,

Associate professor at the department of Cell and Molecular Biology, Karolinska Institutet

Organized by the Laboratories for Chemical Biology Karolinska Institutet (LCBKI) and the Oscar Fernandez-Capetillo Group.


New CBCS Webpage Developed

During early spring 2019, a completely rebuilt CBCS webpage is designed and launched, offering our audience an improved experience in collaborating and interacting with CBCS.

Please stay tuned for updates.

Technology development project published in JoVE


Quantitating the interaction of small molecules with their intended protein target is critical for drug development, target validation and chemical probe validation. Methods that measure this phenomenon without modification of the protein target or small molecule are particularly valuable though technically challenging. The cellular thermal shift assay (CETSA) is one technique to monitor target engagement in living cells. Here, we describe an adaptation of the original CETSA protocol, which allows for high throughput measurements while retaining subcellular localization at the single cell level. We believe this protocol offers important advances to the application of CETSA for in-depth characterization of compound-target interaction, especially in heterogeneous populations of cells.

To cite this: Axelsson, H., Almqvist, H., Seashore-Ludlow, B. Using High Content Imaging to Quantify Target Engagement in Adherent Cells. J. Vis. Exp. (141), e58670, doi:10.3791/58670 (2018).