Unique assays, developed in relevant biological environments is the first step towards investigating small molecules. This work is usually developed within the team of the collaborating investigator. CBCS can assist with this process and also with the highly specialized process of transferring a bioassay from lab-scale to microtiter-based formats, capable of being robustly screened in 96 and 384-well formats. Below are just a few examples of assay technologies and detection methods CBCS has experience with:
High Throughput/High Content Microscopy/Imaging
TR-FRET (HTRF. LANCE)
Luminescence and Absorbance
Our dedicated staff has experience in working with a variety of established cell lines, in addition to primary cells and more exploratory assays environments, such as human neutrophils, human NK cells, mouse erythroid progenitor cells, mouse neurons, primary human cancer cells, iPS cells and organoids.
Our well-equipped and flexible facilities can accommodate medium- and high-throughput screens. Since 2010, we have performed hundreds of screens and smaller projects in our facilities in collaboration with our investigators. These have ranged from studies of isolated biochemical targets, to advanced primary cellular systems, phenotypic and high-content screens, plant biology, and whole organism screening in infectious diseases and cancer.
Performing a high-throughput screen is a daunting task and requires diligent and thorough preparation. CBCS utilizes a flexible screening platform based on multiple work stations providing automated liquid handling, transfer, dispense, and analysis, rather than an integrated full-scale HTS system. This allows for flexibility to accommodate a broad range of screening technologies and sizes.
Learn more about assay development and screening
If you are interested in the basics of high throughput screening (HTS) and want to know more about what different assays there are, we recommend you to register to our web-based 2 ECTS course “Introduction to high-throughput screening”. This course gives you a solid ground when you want to start to develop an assay for your own research question, as well as gives you the necessary terminology used in HTS. The course is entirely web-based and you can study at your own pace.