Pharmacokinetic Profiling of Small Molecules
As part of CBCS, the Uppsala University Drug and Pharmaceutical Profiling Platform (UDOPP) provides unique expertise and a state-of-the art laboratory for absorption, distribution, metabolism, excretion (ADME) investigations and pharmaceutical profiling of molecular probes and drug candidates. We add high scientific value to collaborative chemistry and biological discovery projects, and thereby increase the number of high-quality molecular probes and small molecules for life science applications. Projects can range from brief initiatives to long-term collaborations between the facility and principal investigators at Swedish universities. Projects are preferentially performed as collaborations i.e., the scientific responsibility is shared between UDOPP and the research partners.
Examples of Services provided by UDOPP:
Active cellular uptake and efflux (identification and characterization).
ADME. Absorption, Distribution, Metabolism, Excretion
Biotransformation. Metabolite profiling and identification
Cell Membrane Permeability. Cell monolayer assay.
Computational modeling and in silico models for estimation of ADME properties.
Cytochrome P450 inhibition and induction studies.
Determination and prediction of physico-chemical properties. LogD, LogP and pKa
Determination of solubility (kinetic and thermodynamic) and its pH dependence.
Evaluation of pharmacokinetic (PK) studies.
Human plasma protein binding.
Inhibition of active transport (transporter related drug-drug interactions, DDI).
Integrated cell models for study human drug metabolism and transport processes.
Isolation and characterization of primary human hepatocytes.
Mass spectrometry (MS).
Metabolic enzyme identification studies (reaction phenotyping).
Physiological-based pharmacokinetics (PBPK).
Proteomics. Measurement of abundance of drug metabolizing enzyme and drug transporters in human hepatocytes and in human tissues.